Sheff-CHO ACF is an animal-component-free complex medium supplement containing both nutritional components and protein expression enhancing components. It is suitable for use in classical and chemically defined media.

In batch culture, maximum cell densities in one particular chemically defined medium were doubled over the un-supplemented control. Total protein production was increased almost three-fold. Overall performance increased with dosage with respect to both cell density and productivity. 

Other versions available that include supplementation with trace elements and protein-free